Hormonal regulation of hepatic glycogen synthase phosphatase.
نویسندگان
چکیده
منابع مشابه
Hormonal regulation of glycogen synthase phosphorylation in rabbit skeletal muscle.
Phosphorylation of rabbit skeletal muscle glycogen synthase in vivo in response to hormones was investigated. Methods were developed to purify the synthase without altering its kinetic parameters, thereby indicating constancy of the synthase phosphorylation state. The enzyme from control rabbits had an activity ratio (-glucose-6-P/+glucose-6-P) of 0.24, K, for glucose-6P of 0.17 m ~ , and K , f...
متن کاملCalcium ions and glycogen act synergistically as inhibitors of hepatic glycogen-synthase phosphatase.
We investigated the inhibitory effect of Ca2+ in the micromolar range on the activation of glycogen synthase in crude gel-filtered liver extracts [van de Werve (1981) Biochem. Biophys. Res. Commun. 102, 1323-1329]. The magnitude of the inhibition was highly dependent on the glycogen concentration in the final liver extract. Ca2+ inhibited the activation of purified hepatic synthase b by the G-c...
متن کاملAssociation of glycogen synthase phosphatase and phosphorylase phosphatase activities with membranes of hepatic smooth endoplasmic reticulum
A detailed investigation was conducted to determine the precise subcellular localization of the rate-limiting enzymes of hepatic glycogen metabolism (glycogen synthase and phosphorylase) and their regulatory enzymes (synthase phosphatase and phosphorylase phosphatase). Rat liver was homogenized and fractionated to produce soluble, rough and smooth microsomal fractions. Enzyme assays of the frac...
متن کاملGlycogen synthase kinase 3alpha-specific regulation of murine hepatic glycogen metabolism.
Glycogen synthase kinase 3 comprises two isoforms (GSK-3alpha and GSK-3beta) that are implicated in type II diabetes, neurodegeneration, and cancer. GSK-3 activity is elevated in human and rodent models of diabetes, and various GSK-3 inhibitors improve glucose tolerance and insulin sensitivity in rodent models of obesity and diabetes. Here, we report the generation of mice lacking GSK-3alpha. U...
متن کاملInsulin stimulation of heart glycogen synthase D phosphatase (protein phosphatase).
Insulin rapidly produced an increase in per cent of total heart glycogen synthase in the I form in fed rats. In fasted rats the response was diminished and delayed. In diabetic animals there was no response over the 15-min time period studied. Since synthase phosphatase activity is necessary for synthase D to I conversion, the phosphatase activity was determined in extracts from these groups of...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1981
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)69692-4